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EVs affinity-enrichment (anti-CD8 mAbs). The EVs were enriched from MOLT-3 conditioned media and released by LED exposure from the EV enrichment microfluidic device. The released EVs were subjected to NTA (A), TEM (B), and ddPCR (C). The ddPCR was carried out on 8 genes. Among them, five genes (PLBD1, FOS, MMP9, CA4 and VCAN) are known to be dysregulated as a result of AIS event. See Table S3 for the sequences of the primers used for the ddPCR.
