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. Author manuscript; available in PMC: 2021 Apr 14.
Published in final edited form as: Chem Commun (Camb). 2020 Mar 12;56(29):4098–4101. doi: 10.1039/c9cc09598e

Scheme 1.

Scheme 1.

Structure of the heterobifunctional PC linker and immobilization strategy employing two EDC/NHS coupling reactions. The PC linker is immobilized to carboxylated (UV/O3-oxidized) surfaces via the linker’s primary amine group. Abs are then anchored to the linker’s -COOH handle. After biomarker purification, a visible LED (400–450 nm) cleaves the PC linker. Immobilization reactions are conducted in organic solvent to mitigate NHS ester hydrolysis, which could lead to direct (and non-releasable) Ab conjugation to the -COOH surface.