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. Author manuscript; available in PMC: 2020 Sep 3.
Published in final edited form as: Biochem Biophys Res Commun. 2020 Jul 31;529(4):1101–1105. doi: 10.1016/j.bbrc.2020.06.126

Fig. 3. Analysis of the PGI-lactate axis.

Fig. 3.

(A) Serum L-lactate levels (mM) of healthy donors (n = 5) and NASH patients (n = 14). A 30-min lactate assay was performed using deproteinized serum samples after a 10-fold dilution with dH2O. (B) HepG2 cells were treated with 100 ng/ml recombinant PGI protein for 3 days, following which cell lysates were prepared. Five μg proteins of each cell lysate were used to assay the enzyme activities of glucokinase and GAPDH. (C) Cell culture medium was harvested after 3 days. Culture medium L-lactate concentrations were determined using deproteinized medium samples after a 10-fold dilution with dH2O. Statistical significance is indicated where relevant.