Figure 3. Synchronized Ca²⁺ Transients from Neuron and Astrocyte Populations Elicited by Single Sensory Stimulation (Hind Paw, 3 mA and 0.3 ms).

(A and B) Recorded neuronal and astrocytic Ca²⁺ traces of individual stimulations (m = 20) and their averaged traces (bold curves); the insets show the neuronal and astrocytic GCaMP6f fluorescence images.

(C–E) Statistical comparisons of amplitude, latency, and duration between neuronal and astrocytic GCaMP6f Ca²⁺ fluorescence transients.

(F and G) Traces of Ca²⁺ transients in single-cell ROIs (m = 15) elicited by a sensory stimulation for neuronal and astrocyte populations; small circles (~ϕ30 mm each, m = 15) represent the ROIs (blue for neurons, red for astrocytes) selected for cross-correlation analysis.

(H and I) Cross-correlation charts (i, j = 1,…,m) for neuronal and astrocyte populations and spatiotemporal correlations of neuronal and astrocytic Ca²⁺ transients.

(J) Durations of neuronal and astrocytic Ca²⁺ responses of single cells within the ROIs.

The data are shown as mean ± SEM.