Figure 1.

A diagram illustrating fractionation steps of culture broth of Xenorhabdus hominickii. Organic extracts were collected from hexane (HEX), ethyl acetate (EAX), chloroform (CX), and butanol (BX) organic solvents. The butanol extract was separated using a chromatography column filled with silica gel where a gradient chloroform/methanol mixture with increasing amount of methanol from 100:0 to 0:100 (v/v) was used. The active butanol fractions (F2 and F6) were further separated using preparatory thin-layer chromatography (TLC).