Skip to main content
. 2020 Jun 18;129(1):124–132. doi: 10.1152/japplphysiol.00456.2019

Fig. 5.

Fig. 5.

Mitochondrial-targeted catalase (MCAT) suppresses mitochondrial oxidative stress. A: representative Western blots of catalase and citrate synthase (CS; used as a loading control) in isolated skeletal muscle mitochondria from wild-type (WT) and MCAT mice with or without hindlimb unloading (HU). B: superoxide dismutase 2 (SOD2) protein abundance in skeletal muscle isolated mitochondria from WT (open bars) and MCAT (black bars) mice with or without HU (WT non-HU, n = 3; MCAT non-HU, n = 3; WT HU, n = 3; MCAT HU, n = 3). C: glutathione peroxidase 4 (GPx4) protein abundance in isolated skeletal muscle mitochondria (WT non-HU, n = 3; MCAT non-HU, n = 3; WT HU, n = 3; MCAT HU, n = 3). D: rate of mitochondrial H2O2 production in response to 10 mM succinate (WT non-HU, n = 5; MCAT non-HU, n = 5; WT HU, n = 7; MCAT HU, n = 7). E and F: representative images and quantification of muscle 4-hydroxynonenal (4-HNE) at ∼50 and ∼20 kDa in whole skeletal muscle tissues (WT non-HU, n = 3; MCAT non-HU, n = 3; WT HU, n = 3; MCAT HU, n = 3). *Significantly different from WT (P < 0.05); #significantly different from non-HU (P < 0.05). Values are means ± SE.