Skip to main content
. 2020 Sep;98(3):267–279. doi: 10.1124/mol.120.000020

Fig. 9.

Fig. 9.

Effect of CORM2 on activity, protein levels, and HMM species formation in HuH7 cells. (A) HuH7-2B6V5 cell lines were treated with medium (Con), DPTA (D, 500 mM), CORM2 (1 mM), or DPTA/CORM2 for 2 hours. CYP2B6 activities were measured as described in the text, and total cell lysates were used for immunoblotting with antibodies to the V5 tag and GAPDH. Values are means and 95% CI, n = 4. (B) Representative immunoblotting image showing significant HMM species accumulation with CORM2 treatments from three independent experiments. (C) HuH7-2B6V5 cells were transiently transfected with pCMV-HA-ubiquitin, they were treated with DPTA or CORM2 for 1 hour, and cell lysates were subjected to immunoprecipitation with anti-V5 agarose. The lysates and immunoprecipitates were subjected to immunoblotting with anti-V5. (D) The immunoprecipitates were subjected to Western blotting with HA antibodies. Con, control; MW, molecular weight.