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. 2020 Sep 3;20:838. doi: 10.1186/s12885-020-07275-5

Fig. 2.

Fig. 2

SNHG3 silencing effectively inhibited BC cells proliferation, invasion and migration. Two siRNAs targeted SNHG3 and scramble siRNA were transfected into MCF-7 and HCC1937 cells. a. RT-qPCR was performed to validate siRNA transfection. MCF-7 and HCC1937 cell biological behaviors were detected with EdU staining (b); BC cell proliferation detected by MTT proliferation assay (c) and colony formation assays (d); E. MCF-7 and HCC1937 cells migrating from upper Transwell chambers into lower ones, without Matrigel (× 200); f. MCF-7 and HCC1937 cells invading from Matrigel-coated upper Transwell chambers into lower ones (× 200); g. Western blot analysis was carried out to determine E-cadherin and N-cadherin protein levels (representative images were shown, full-length gels are presented in Supplementary Figure 1). Three independent experiments were performed. Data are expressed as mean ± standard deviation; one-way ANOVA and Sidak’s multiple comparisons test was used to determine statistical significance, or two-way ANOVA and Tukey’s multiple comparisons test was used, *p < 0.05, **p < 0.01