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. 2020 Sep 3;19:319. doi: 10.1186/s12936-020-03374-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Proportion of P. falciparum and P. vivax infections detected by qPCR versus us-qPCR by country. Proportion of P. falciparum (a) and P. vivax infections (b) positive by species-specific 18S rRNA qPCR among all samples positive by any qPCR assay (standard qPCR or us-qPCR) and parasite densities of P. falciparum (c) and P. vivax infections (d) detected by 18S rRNA qPCR versus only by us-qPCR. White circles: infection detected by standard qPCR; black circles: infection detected only by us-qPCR. ¹Owing to exhaustion of DNA, the parasite densities of 5 Brazilian samples (white circles) were quantified by 18S rRNA qPCR