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. 2020 Sep 3;20:428. doi: 10.1186/s12935-020-01487-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — EIF3J-AS1 acts as a molecular sponge for miR-1343-3p in glioma. a, b Subcellular fractionation and FISH assays were combined to explore the location of EIF3J-AS1. c RNA pull down was performed to study the potential interaction between candidate miRNAs and EIF3J-AS1. d The expression of miR-1343-3p was detected in glioma cells and normal one. e RIP assay revealed that EIF3J-AS1 had the potential to bind to Ago2. f RNA pull down validated the interaction between EIF3J-AS1 and miR-1343-3p. g Putative and mutate miR-1343-3p binding sites with EIF3J-AS1 were displayed. h MiR-1343-3p expression was up-regulated by transfection of miR-1343-3p mimics. i Dual-luciferase reporters showed that miR-1343-3p mimics decreased the luciferase activity of vector containing EIF3J-AS1-WT. j, k Colony formation and EdU assays were performed to evaluate cell proliferation after up-regulating miR-1343-3p. l, m TUNEL and Caspase 3/8/9 activity assays were used to explore glioma cells apoptotic ability after up-regulating miR-1343-3p. **P < 0.01