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. 2020 Jun 28;19(15):1928–1940. doi: 10.1080/15384101.2020.1783934

Figure 3.

Figure 3.

miR-203 targets LXR-α and PPAR-γ to negatively regulate their expression. (a) miR-203 expression in psoriasis lesion and nonlesion tissues examined by real-time PCR (n = 12). (b) miR-203 expression determined in HaCaT cells in the presence or absence of IL-22 (n = 5). (c) miR-203 expression in HaCaT cells after transfection of miR-203 or anti-miR-203 vector, as confirmed by real-time PCR. miR-NC and anti-NC were transfected as negative control for miR-203 and anti-miR-203, respectively. (d) The protein levels of LXR-α and PPAR-γ in response to miR-203 overexpression or inhibition determined in HaCaT cells using Immunoblotting (n = 3). (e) A luciferase reporter assay was performed by constructing wild-type and mutant LXR-α and PPAR-γ luciferase reporter vectors. These vectors were cotransfected in 293 T cells with miR-203 or anti-miR-203 and the luciferase activity was determined (n = 3). **P < 0.01, ##P < 0.01, compared to miR-NC (negative control) or anti-NC group (negative control).