Figure 5.

Dynamic effects of miR-203 and LXR-α on keratinocytes HaCaT cells were treated with IL-22 to generate a psoriasis-like dermatitis model in vitro. The cells were cotransfected with miR-203 and the LXR-α-overexpressing vector (miR-NC and Vector were used as negative controls, respectively), and examined for (a) cell viability using the MTT assay; (b) DNA synthesis capacity by EdU assay; (c) the mRNA expression of KRT5, KRT10, KRT1, and KRT14 using real-time PCR; and (d) the protein levels of LXR-α, ki67, KRT1, KRT10, KRT5, and KRT14 using immunoblotting. *P < 0.05, **P < 0.01, compared to control group; ##P < 0.01, compared to the miR-NC (negative control) + LXR-α group.