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. 2020 Jun 28;19(15):1928–1940. doi: 10.1080/15384101.2020.1783934

Figure 6.

Figure 6.

Dynamic effects of miR-203 and PPAR-γ on keratinocytes HaCaT cells were treated with IL-22 to generate a psoriasis-like dermatitis model in vitro and cotransfected with miR-203 and PPAR-γ-overexpressing vector (miR-NC and empty vector were used as negative controls, respectively), and examined for (a) cell viability using MTT assay; (b) DNA synthesis capacity by EdU assay; (c) the mRNA expression of KRT5, KRT10, KRT1, and KRT14 using real-time PCR; (d) the protein levels of PPAR-γ, ki67, KRT1, KRT10, KRT5, and KRT14 using immunoblotting. *P < 0.05, **P < 0.01, compared to control group; ##P < 0.01, compared to miR-NC (negative control) + PPAR-γ group.