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CMS does not trigger ribophagy in hTM cells. (A) hTM cells were transfected with siATG5,7 or siNC for 48 h and then subjected to CMS (8% elongation, 24 h). Protein levels of LC3, RPL26 and RPS15A were evaluated by WB. Cells cultured for 24 h in HBSS served as positive control. (B) Band intensities were normalized with ACTB and fold expression calculated. Data are shown as the mean ± S.D. (n = 3), two-tailed unpaired Student’s t-test. ns: not significant.
