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. Author manuscript; available in PMC: 2020 Sep 17.
Published in final edited form as: Immunity. 2020 Mar 10;52(3):528–541.e7. doi: 10.1016/j.immuni.2020.02.005

Figure 2. ILC2 homeostasis in the proximal small intestine is leukotriene-independent and minimally requires IL-25 and IL-33.

Figure 2.

(A) Quantification of IL-13 (S13) expression by ILC2s (CD45+;Lineage−;IL-17RB+) in the proximal (first 5cm) SI of naïve mice. (B) Mean fluorescence intensity (MFI) of KLRG1 expression by ILC2s. (C) Quantification of MFI in (B). (D-F) mRNA sequencing of ILC2s of the indicated genotype sorted from the proximal SI of naïve mice. (D) PCA of top 500 differentially expressed genes (DEG). (E) Venn diagram depicting DEGs for each genotype as compared to wildtype (FDR <.05, row mean >10). (F) Volcano plots depicting DEGs as compared to wildtype (FDR <.05, row mean >10). Red = log2 fold change > 1, blue = log2 fold change < 1. In (A-D) each symbol represents an individual mouse pooled from two or more experiments. Samples in D were analyzed in one sequencing run. *p < 0.05, **p < 0.01, ***p < 0.001 by one way ANOVA (A, C) with comparison to S13. n.s., not significant. Graphs depict mean + SEM. See also Figure S2 and Tables S1–4.