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. Author manuscript; available in PMC: 2020 Sep 17.
Published in final edited form as: Immunity. 2020 Mar 10;52(3):528–541.e7. doi: 10.1016/j.immuni.2020.02.005

Figure 4. Tuft-ILC2 circuit activation and helminth clearance are delayed in the absence of cysteinyl leukotrienes.

Figure 4.

(A) Tuft cell frequency in the proximal (first 10cm) SI of mice infected with H. polygyrus (H.p.) for 4 days. DCLK1 (yellow) marks tuft cells. DAPI (blue) marks nuclei. Scale bar: 50μm. (B) Quantification of tuft cells in (A). (C) Tuft cell frequency in the proximal SI after 14 days of H.p. infection. (D-E) Tuft cell frequency in the proximal SI of the indicated genotypes after 4 days of H.p. infection. (F) Tuft cell frequency in the proximal and distal (last 10cm) SI of wildtype mice at the indicated time points post-N. brasiliensis (N.b.) infection. (G) Representative images of proximal SI on day 5 post-N. b. infection. Scale bar: 50μm. (H) Quantification of tuft cells in (G). (I) Tuft cell frequency in the distal SI after 7 days of N.b. infection. (J) Goblet cells identified in the jejunum (10-20cm from stomach) by alcian blue staining 7 days after infection with N.b. Representative images are shown. Scale bar: 50μm. (K-L) Quantification of goblet cell (K) number and (L) size in (J). (M) Worm burden across the entire SI at the indicated time points post-N.b. infection. In (B)-(E), (H)-(I), and (K)-(M) each symbol represents an individual mouse pooled from two or more experiments. In (F) each symbol represents the average of five mice pooled from two experiments, d.p.i., days post infection. *p < 0.05, **p < 0.01, ***p < 0.001 by Mann-Whitney (C, H, K-L), by multiple t tests (B, F, I, M), or by one way ANOVA (D-E) with comparison to Wt(B6). n.s., not significant. Graphs depict mean + SEM. See also Figure S4.