KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| CD326 (EpCAM) APC, clone G8.8 | BioLegend | Cat#118214, RRID: AB_1134102 |
| Human CD4 PE, clone RPA-T4 | BioLegend | Cat#300508, RRID: AB_314076 |
| CD45 BV605, clone 30-F11 | BioLegend | Cat#103155, RRID: AB_2650656 |
| CD45 BV650, clone 30-F11 | BioLegend | Cat#103151, RRID: AB_2565884 |
| KLRG1 PECy7, clone 2F1/KLRG1 | BioLegend | Cat#138416, RRID: AB_2561736 |
| CD3 Percp-cy5.5, clone 145-2C11 | BioLegend | Cat#100328, RRID: AB_893318 |
| CD4 Percp-cy5.5, clone RM4-5 | BioLegend | Cat#100540, RRID: AB_893326 |
| CD5 Percp-cy5.5, clone 53-7.3 | BioLegend | Cat#100624, RRID: AB_2563433 |
| CD8 Percp-cy5.5, clone 53-6.7 | BioLegend | Cat#100724, RRID: AB_389326 |
| CD11b Percp-cy5.5, clone M1-70 | BioLegend | Cat#101228, RRID: AB_893232 |
| CD11 b AlexaFluor 700, clone M1-70 | BioLegend | Cat#101222, RRID: AB_493705 |
| CD19 Percp-cy5.5, clone 1D3/CD19 | BioLegend | Cat#152406, RRID: AB_2629815 |
| FceRI Percp-cy5.5, clone MAR-1 | BioLegend | Cat#134320, RRID: AB_10641135 |
| NK1.1 Percp-cy5.5, clone PK136 | BioLegend | Cat#108728, RRID: AB_2132705 |
| Siglec F APC-Cy7, clone E50-2440 | BD Pharmingen | Cat#565527, RRID: AB_2732831 |
| CD24 Percp-cy5.5, clone M1/69 | BioLegend | Cat#101824 RRID: AB_1595491 |
| Ki67 AlexaFluor 488, clone 16A8 | BioLegend | Cat#652417 RRID: AB_2564236 |
| Rabbit anti-DCAMKL1 | Abcam | Cat#ab31704, RRID: AB_873537 |
| F(ab’)2 goat anti-rabbit IgG AF594 | Invitrogen | Cat#A11072, RRID: AB_2534116 |
| Rabbit anti-5-LO | Sigma Aldrich | Cat# SAB1410449 RRID: AB_2819149 |
| Thy1.2 (CD90.2) BV605, clone 53-2.1 | Biolegend | Cat#140318 RRID: AB_2650924 |
| ST2(Il1rl1) biotin, clone DJ8 | MD Bioscience | Cat#101001B RRID: AB_947551 |
| Rabbit anti-BrdU-AF594 | Abcam | Cat#ab220076 RRID: AB_2819150 |
| Rabbit anti-NFATC2 (NFAT1), clone D43B1 | Cell Signaling Technology | Cat#5861T RRID: AB_10834808 |
| Rabbit anti-pEGFR (Y1068), clone EP774Y | Abcam | Cat#ab205827 RRID: AB_732110 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Recombinant IL-13 | Peprotech | Cat#210-13 |
| Recombinant IL-25 | R & D Systems | Cat#1399-IL-025 |
| Recombinant IL-33 | R & D Systems | Cat#3626-ML-010 |
| Recombinant IL-7 | R & D Systems | Cat#h407-ML-005 |
| Strepavidin PE/Dazzle | Biolegend | Cat#405248 |
| Vectashield with DAPI | Vector Laboratories | Cat#H-1200 |
| DNase I | Sigma Aldrich | Cat#D4513 |
| LiberaseTM | Sigma Aldrich | Cat#5401127001 |
| TSA Blocking reagent | Perkin Elmer | Cat#FP1020 |
| DAPI | Roche | Cat#10236276001 |
| Fc Block | BD Biosciences | Cat#553141 |
| Accutase | Corning | Cat# 25058C1 |
| Recombinant murine EGF | Peprotech | Cat# 31509A |
| Y27632 | Stemcell Technologies | Cat# 72302 |
| SB431542 | Stemcell Technologies | Cat# 72232 |
| Leukotriene C4 | Cayman Chemical | Cat# 20210 |
| Leukotriene D4 | Cayman Chemical | Cat# 20310 |
| Leukotriene E4 | Cayman Chemical | Cat# 20410 |
| Leukotriene B4 | Cayman Chemical | Cat# 20110 |
| Montelukast | Cayman Chemical | Cat# 10008318 |
| BrdU | BioLegend | Cat# 423401 |
| Sodium succinate hexahydrate | Alfa Aesar | Cat#41983 |
| Critical Commercial Assays | ||
| FoxP3 Transcription Factor Staining Kit | ThermoFisher | Cat#00-5523-00 |
| RNeasy Plus Micro Kit | Qiagen | Cat#74034 |
| SuperScript VILO cDNA Synthesis Kit | Invitrogen | Cat#11766050 |
| SMARTseq V4 Ultra Low Input RNA Kit | Takara | Cat# 634894 |
| Cysteinyl Leukotriene Express ELISA Kit | Cayman Chemical | Cat#10009291 |
| Deposited Data | ||
| RNAseq data files | This paper | GEO: GSE144956 |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6J | Jackson Laboratory | Cat# 000664 |
| Mouse: B6.129P2-Trpm5tm1Dgen/J | Jackson Laboratory | Cat# 005848 |
| Mouse: B6.129S2-Alox5tm1Fun/J | Jackson Laboratory | Cat# 004155 |
| Mouse: B6.Cysltr1tm1Ykn/J | Jackson Laboratory | Cat#030814 |
| Mouse: B6.Cg-Tg(Vil1-cre)1000Gum/J | Jackson Laboratory | Cat# 021504 |
| Mouse: B6.129P2-Lyz2tm1(cre)Ifo/J | Jackson Laboratory | Cat# 004781 |
| Mouse: B6.Il25Flare25/Flare25 | von Moltke et al., 2016 | N/A |
| Mouse: B6.Il13Smart13/Smart13 | Liang et al., 2012 | N/A |
| Mouse: B6.Arg1YARG/YARG | Reese et al. 2007 | N/A |
| Mouse: B6.Pou2f3−/− | Provided by M. Anderson: Project ID #VG18280 | N/A |
| Mouse: B6.Tg(Cpa3-cre)3Glli | Provided by A. Piliponsky | N/A |
| Mouse: B6.Il25−/− | Provided by A. McKenzie, via R. Locksley | N/A |
| Mouse: B6.Il1rl1−/− | Provided by S. Akira, via R. Locksley | N/A |
| Mouse: Nmur1tm1.1(KOMP)Vlcg | Provided by V. Kuchroo | N/A |
| Mouse: B6.Cysltr1−/− | Provided by N. Barrett | N/A |
| Mouse: B6.Cysltr2−/− | Provided by N. Barrett | N/A |
| Mouse: Alox5-flox | This paper | N/A |
| Mouse: Pou2f3-creErt2-eGFP | This paper | N/A |
| Oligonucleotides | ||
| Rps17qRT-PCR primers: 5’-CGCCATTATCCCCAGCAAG-3’, 5’-TGTCGGGATCCACCTCAATG-3’ | Von Moltke et al., 2017 | N/A |
| Cysltr1 qRT-PCR primers: 5’-TTGAGCCTCCACAGAACAATC-3’, 5’-TTCCTACGACTTGGCATGTTTT-3’ | Von Moltke et al., 2017 | N/A |
| Cysltr2 qRT-PCR primers: 5’-TGTCACCAGTGTCAGGAGTG-3’, 5’-ACTTTTGAGGACTCAGCTCCAA-3’ | https://pga.mgh.harvard.edu/primerbank/ | PrimerBank ID# 19526902a1 |
| Ltb4r1 qRT-PCR primers: 5’-CTGATCTGCGCTCCGAACTAT-3’, 5’-TGCGCCCGATGTCAGAGTA-3’ | https://pga.mgh.harvard.edu/primerbank/ | PrimerBank ID# 161760677C3 |
| Ltb4r2 qRT-PCR primers: 5’-ATGTCTGTCTGCTACCGTCC-3’, 5’-AGCTCCATACTACGAAGCCAT-3’ | Von Moltke et al., 2017 | N/A |
| Oxgr1 qRT-PCR primers: 5’-CAGCCAGTGATTCGGATTTCC-3’, 5’-GCTGGTCAGATACAGCAAGTC-3’ | Von Moltke et al., 2017 | N/A |
| Il25 qRT-PCR primers: 5’-ACAGGGACTTGAATCGGGTC-3’, 5’-TGGTAAAGTGGGACGGAGTTG-3’ | Von Moltke et al., 2016 | N/A |
| Pla2g4a qRT-PCR primers: 5’-TGGTGGGATTCTCTGGTGTGA-3’, 5’-GGAAAATCGGGGTGAGAGTACA-3’ | https://pga.mgh.harvard.edu/primerbank/ | PrimerBank ID# 133891677c3 |
| Alox5 qRT-PCR primers: 5’-ACTACATCTACCTCAGCCCATT-3’, 5’-GGTGACATCGTAGGAGTCCAC-3’ | https://pga.mgh.harvard.edu/primerbank/ | PrimerBank ID# 886333a1 |
| Alox5ap qRT-PCR primers: 5’-AGCATGAAAGCAAGGCGCATA-3’, 5’-GTACGCATCTACGCAGTTCTG-3’ | https://pga.mgh.harvard.edu/primerbank/ | PrimerBank ID# 33563242a1 |
| Ltc4s qRT-PCR primers: 5’-ATGAAGGACGAAGTGGCTCTT-3’, 5’-CCTGTAGGGAGAAGTAGGCTTG-3’ | https://pga.mgh.harvard.edu/primerbank/ | PrimerBank ID# 20380551a1 |
| Lta4h qRT-PCR primers: 5’-CTGACTTTGGACACAAAAGACCT-3’, 5-AGATTTCCATCGGTGACCCTT-3’ | https://pga.mgh.harvard.edu/primerbank/ | PrimerBank ID# 116734869C2 |
| Software and Algorithms | ||
| GraphPad Prism 7 | GraphPad Software | N/A |
| FlowJo 10.4.2 | Tree Star | https://www.flowjo.com/solutions/flowjo/downloads |