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. 2019 Oct 2;16(7):1279–1295. doi: 10.1080/15548627.2019.1668606

Figure 4.

Figure 4.

PPX promotes phosphorylation of PRKAA (Thr172) and RPTOR (Ser792), and strengthens RPTOR-MTOR interaction through DRD3 but not DRD2. (A and B) Western blot for total and phosphorylated forms of PRKAA at Thr172 (p-PRKAA) and RPTOR at Ser792 (p-RPTOR). (C) Immunoprecipitation for MTOR (IP MTOR) followed by western blot for RPTOR and p-RPTOR in DRD3- and DRD2-HEK cells. PRKAA and RPTOR phosphorylation, and the interaction RPTOR (p-RPTOR)-MTOR only increased in DRD3-HEK cells, and this effect was blocked by co-treatment with NGB2904.