Figure 5.

Cell viability is affected by direct MTOR inhibitors but not by DRD3-induced MTOR inhibition. (A) MTT assay in DRD3-HEK cells treated with rapamycin, torin 1 and PPX (0.01–10 µM, 72 h). Cell viability was significantly reduced in DRD3-HEK cells treated with rapamycin and torin 1 but not in cells treated with PPX. (B) Quantitative analysis of malonildialdehyde (MDA) in the striatum of WT mice treated with PPX (0.15 mg/kg/d/6 d) or CCI-779 (20 mg/kg/48 h i.p. x4). Striatal MDA levels were significantly increased in mice treated with CCI-779 but not in those treated with PPX.