Figure 2.

Administration of the TSPO ligand PK11195 into the 3V reduces food intake and stimulates energy expenditure in NCD- and HFD-fed mice. Mice were fed NCD or HFD for 4 weeks, and PK11195 or vehicle (2% ethanol) was injected into the 3V through a cannula. (A–F) Body weight change (A and D), food intake (B and E), and hypothalamic Agrp, Npy, Pomc and Tspo mRNA expression (C and F) in mice fed NCD (A–C; n = 10) or HFD (D–F; n = 5). (G–L) RER (G), VCO₂ (H), VO₂ (I), heat production (J), locomotor activity x axis (XTOT) (K), and z axis (ZTOT) (L). (NCD, n = 12; HFD, n = 8). Data are mean ± SEM, *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001 vs. NCD vehicle; ^#P ≤ 0.05, ^##P ≤ 0.01, and ^###P ≤ 0.001 vs. HFD vehicle as determined by Student’s t-test.