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. 2019 Aug 30;16(7):1200–1220. doi: 10.1080/15548627.2019.1659616

Figure 6.

Figure 6.

Ablation of Tspo in tanycytes decreases food intake and increases energy expenditure in HFD-fed mice. (A) Strategy to generate conditional tanycyte-specific Tspo knockout mice by viral injection of AAV-Rax-Cre-GFP into the 3V in Tspofl/fl mice. (B) Immunostaining for TSPO (red) and VIM (magenta) with Hoechst (blue) staining in GFP-positive ventral tanycytes of AAV-Rax-Cre-GFP-injected Tspofl/fl mice. (C) High-magnification micrographs showing GFP (green), Hoechst (blue), and TSPO (red) in ventral tanycytes of Tspofl/fl mice injected with AAV-Rax-Cre-GFP or AAV-Rax-GFP. (D–K) Body weight change (D), accumulated food intake (E), RER (F), VCO2 (G), VO2 (H), heat production (I), XTOT (J), and ZTOT (K) of Tspofl/fl mice fed HFD for 4 weeks after injection with AAV-Rax-Cre-GFP or AAV-Rax-GFP into the 3V (n = 9). Data are mean ± SEM, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 vs. AAV-Rax-GFP, as determined by two-way ANOVA (D and E) or Student’s t-test (F-I).