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. 2020 Apr 19;19(11):1275–1284. doi: 10.1080/15384101.2020.1746874

Figure 4.

Figure 4.

miR-375 expressed in hASMCs participated in regulating the cellular functions of hMVECs. Followed with the transfection of miR-375 mimic and inhibitor, hASMCs were treated with cytokines (10 ng/mL IFN-γ, IL-1β and TNF-α) and hMVECs were cultured in the conditioned media for 24 h. (a) The relative miR-375 expression level of asthmatic and healthy tissues of patients was determined using RT-qPCR. (b and c) The results of RT-qPCR showed the expressions of miR-375 and YAP1 in cytokine-stimulated hASMCs after mimic or inhibitor transfections. (d) Using CCK-8 method, we measured the proliferation ability of hMVECs. (e) Transwell assay supplied the migrated cell numbers of CM cultured hMVECs. (f) The quantified branching points of hMVECs under different treatments of Matrigel assay. (g) The expressions of target proteins in hASMCs were measured by Western blot assay. All data were derived from at least three repeated experiments, as mean ± SEM. * stands for a P value less than 0.05 compared with non-treated group. # P < 0.05 versus group of cytokines-stimulation only.