Figure 5.

The alleviation of hMVEC cell dysfunction induced by YAP1 knockdown in hASMCs was modulated by miR-375. Firstly, hASMCs were transfected by miR-375 mimic or/and pcDNA-YAP1; subsequently, hASMCs were treated with cytokines; finally, hMVECs were cultured for 24 h in hAMSC-conditioned media. (a) The determination of YAP1 expression in hASMC was executed using RT-qPCR. (b) CCK-8 assay showed the cell proliferation of hMVECs. (c) Transwell assay was conducted for the cell migration of hMVECs. (d) Matrigel method supplied the quantification of branching points of hMVECs. (e) Western blot assay presented the expressions of target proteins in hASMCs. Triplicate independent experiments had been done and the results were presented as mean ± SEM. * P < 0.01 versus non-treated group. ** stands for P < 0.01 compared with non-treated group. ^# P < 0.05 versus group of cytokines-stimulation only.