Figure 2.

Overexpression of hsa_circ_0009910 caused changes in cellular phenotypes associated with proliferation and motility. (a) qRT-PCR analysis of hsa_circ_0009910 levels in SKOV3 cells transfected with pcDNA3.1 carrying genomic sequence coding hsa_circ_0009910 or hsa_circ_0009910 siRNA. Vector and scramble served as control. GAPDH was used for qRT-PCR normalization. (b) CCK-8 assay examined the percentage of viable cells after transfection. (c–d) Western blot analysis was carried out for molecular change in cyclin D1, CDK4 and CDK6. (e–f) Migratory and invasive cells were counted under flow cytometry after crystal violet staining. (g–h) Western blot analysis was used for the detection of MMP-2 and MMP-9. β-Actin was used for protein normalization. Error bars were standard deviation from three independent experiments. P-values were calculated using student’s t test or one-way analysis of variance. *P < 0.05, **P < 0.01, ***P < 0.001.