Figure 1.

MiR-425-5p promotes proliferation, migration, invasion and EMT of CRC cells. To reveal the biological function of miR-425-5p, NC mimic or miR-425-5p mimic and NC inhibitor or miR-425-5p inhibitor were transfected into SW480 and LOVO cells, respectively. At 24 h or 48 h after transfection, cells in each group were gathered for the following experiments. (a) RT-qPCR was carried out to detect miR-425-5p expression. (b) CCK-8 assay was performed to evaluate cell viability at 24 h, 48 h and 72 h. (c) Flow cytometry was performed to detect the distribution of cell cycle. (d) Wound healing assay revealed cell mobility and images were taken at 0 h and 24 h. (e) Cell invasion capability was examined using transwell assay. (f) Western blot analysis showed the expression of Fibronectin, E-cadherin, N-cadherin and Vimentin. (g) Relative expression of Fibronectin was evaluated by immunofluorescence staining. Data are presented as the mean ± SD of three independent samples. *p < 0.05, **p < 0.01, ***p < 0.001 versus NC mimic; ^##p < 0.01, ^###p < 0.001 versus NC inhibitor