Figure 2.

MiR-425-5p induces the activation of β-catenin signaling pathway. After transfection with NC mimic, or miR-425-5p mimic, or NC inhibitor, or miR-425-5p inhibitor for 24 h or 48 h, western blot and immunofluorescence assays were performed to reveal the effect of miR-425-5p on β-catenin signaling pathway and downstream target genes. (a-b) Relative expression of β-catenin in cytoplasm and nucleus was detected using western blot analysis. (c) Immunofluorescence staining revealed β-catenin level. (d) The protein expression of c-myc, Cyclin D1 and MMP7 was measured by western blot assay. Data are presented as the mean ± SD of three independent samples. ***p < 0.001 versus NC mimic; ^##p < 0.01, ^###p < 0.001 versus NC inhibitor