Figure 3.

MiR-425-5p directly targets CTNND1. The 3ʹ untranslated region (UTR) of CTNND1 of wild type (WT) or mutant type (Mut) was cloned into luciferase reporter vector and then co-transfected with NC mimic, or miR-425-5p mimic, or NC inhibitor, or miR-425-5p inhibitor into 293 T cells. (a) Predicted miR-425-5p target sequences in CTNND1 3ʹ UTR, and relative luciferase activity was detected. (b) The mRNA expression of CTNND1 was detected by RT-qPCR. (c) The protein expression of CTNND1 was detected by western blot. Data are presented as the mean ± SD of three independent samples. ^$$p < 0.01 versus CTNND1 3ʹ UTR (site 1-WT) + NC mimic; ^@p < 0.05 versus CTNND1 3ʹ UTR (site 2-WT) + NC mimic; ***p < 0.001 versus NC mimic; ^###p < 0.001 versus NC inhibitor