Figure 4.

Knockdown of CTNND1 inhibits the effect of miR-425-5p inhibitor on CRC cells. After co-transfection of miR-425-5p inhibitor with siNC or siCTNND1 into LOVO cells for 24 h or 48 h, cells were collected for subsequent experiments. (a) CCK-8 assay was performed to detect cell viability at 24 h, 48 h and 72 h. (b) The percentage of LOVO cells in each cell cycle phase was analyzed by flow cytometry. (c-d) The migration and invasion capacities were determined using wound healing and transwell assays. (e) Western blot assay revealed relative expression of E-cadherin, Fibronectin, and β-catenin. Data are presented as the mean ± SD of three independent samples. ^{^}p < 0.05, ^{^^}p < 0.01, ^{^^^}p < 0.001 versus miR-425-5p inhibitor+siNC