Figure 5.

MiR-425-5p promotes the growth and metastasis of CRC tumors. Male BALB/c nude mice of 6-week old were subcutaneously inoculated with 1 × 10⁶ LOVO cells infected with anti-miR-425-5p or anti-miR-NC for the study of tumorigenesis; or they were anesthetized, fixed and the left upper side of mouse abdomen was cut to expose spleen with inoculation of 1 × 10⁶ LOVO cells infected with anti-miR-425-5p or anti-miR-NC for the study of liver metastasis. After treatment for 30 or 35 days, the mice were euthanized and tumor tissues were weighed, photographed. (a) Tumor volume was calculated every 5 days for a total of 30 days. (b) In tumorigenesis experiment, tumor tissues were photographed at 30 days post-inoculation. (c) In liver metastasis experiment, liver tissues were photographed at 35 days post-inoculation. (d) Histological changes in the transplanted livers were observed using hematoxylin and eosin (H&E) staining, and the number of nodules was counted. Data are presented as the mean ± SD of six independent samples. ^&p < 0.05, ^&&p < 0.01, ^&&&p < 0.001 versus anti-miR-NC