Table 2.
Conditions of the antibody incubation
| Primary antibody |
Dilution ratio | Incubation condition | Secondary antibody | Dilution ratio | Incubation condition |
|---|---|---|---|---|---|
| Fibronectin | 1: 1000 | 4°C overnight | Goat anti-rabbit IgG-HRP | 1: 5000 | 37°C 40 min |
| E-cadherin | 1: 10,000 | 4°C overnight | Goat anti-rabbit IgG-HRP | 1: 5000 | 37°C 40 min |
| N-cadherin | 1: 5000 | 4°C overnight | Goat anti-rabbit IgG-HRP | 1: 5000 | 37°C 40 min |
| Vimentin | 1: 2000 | 4°C overnight | Goat anti-rabbit IgG-HRP | 1: 5000 | 37°C 40 min |
| β-catenin | 1: 5000 | 4°C overnight | Goat anti-rabbit IgG-HRP | 1: 5000 | 37°C 40 min |
| c-myc | 1: 2000 | 4°C overnight | Goat anti-rabbit IgG-HRP | 1: 5000 | 37°C 40 min |
| Cyclin D1 | 1: 1000 | 4°C overnight | Goat anti-rabbit IgG-HRP | 1: 5000 | 37°C 40 min |
| MMP7 | 1: 500 | 4°C overnight | Goat anti-rabbit IgG-HRP | 1: 5000 | 37°C 40 min |
| CTNND1 | 1: 1000 | 4°C overnight | Goat anti-rabbit IgG-HRP | 1: 5000 | 37°C 40 min |
| Histone H3 | 1: 500 | 4°C overnight | Goat anti-rabbit IgG-HRP | 1: 5000 | 37°C 40 min |
| β-actin | 1: 2000 | 4°C overnight | Goat anti-mouse IgG-HRP | 1: 10000 | 37°C 40 min |