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. 2020 Apr 19;19(11):1363–1377. doi: 10.1080/15384101.2020.1750257

Figure 2.

Figure 2.

GAS5 regulates H/R-induced cardiomyocyte proliferation and apoptosis. (a) The qPCR analysis of expressions of lncRNA-GAS5, miR-21, PDCD4 and PTEN in H9c2 cells induced by hypoxia for 4–20 h followed by reoxygenation for 3 h. (b) The qPCR analysis of lncRNA-GAS5 expression after overexpression of GAS5 (OE-GAS5) or knockdown of GAS5 (shGAS5). (c) Cell survival assessed by MTT method in H9C2 cells. (d) Cell cycle detection by flow cytometry. (e) Cell apoptosis analysis by flow cytometry under different conditions. (f) Representative images of western blot showing the protein expressions of cleaved caspase-9, BCL-2 and BAX in the cells under different conditions. GAPDH was used as a loading control. Data are presented as the mean ± SD from at least three independent experiments. OE-GAS5: overexpression of GAS5; OE-NC: overexpression of empty plasmid; shGAS5: knockdown of GAS5 with shRNA; shNC: shRNA negative control. *P < 0.05, **P < 0.01 and ***P < 0.001.