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. 2020 Apr 19;19(11):1363–1377. doi: 10.1080/15384101.2020.1750257

Figure 3.

Figure 3.

LncRNA GAS5 directly targets miR-21. (a) MiR-21 binding sites in the lncRNA GAS5. Thirteen nucleotides (bold) mutated in luciferase reporter plasmids carrying GAS5. (b) Dual luciferase reporter assay in H9c2 cells transfected with miR-NC or miR-21 mimics followed by the introduction of GAS5-WT or GAS5-MUT. (c) RIP analysis of GAS5 and miR-21 immunoprecipitated by anti-Ago2. The anti-IgG as a negative control. (d) The qPCR analysis of miR-21 level in the cells transfected with OE-GAS5 or shGAS5. Data are presented as the mean ± SD from at least three independent experiments. OE-GAS5: overexpression of GAS5; OE-NC: overexpression of empty plasmid; shGAS5: knockdown of GAS5 with shRNA; shNC: shRNA negative control. *p < 0.05 and **p < 0.01.