Figure 4.

Vac8 inhibits Atg13[571–700] liposome binding. (A,B) Atg13[571–700], Vac8 [10–515] and a mixture of both proteins were incubated with Folch liposomes in buffer containing 50 mM NaCl and subjected to centrifugation. Pellet and supernatant fractions were collected and analyzed by western blot. The percent of Atg13[571–700] in the pellet was quantified using densitometry. (C,D) Same as A,B except performed in the presence of 150 mM NaCl. Error bars indicate the standard deviation of 4 independent experiments. Statistical analysis by ANOVA: *** P < 0.001.