Skip to main content
. 2019 Nov 12;16(8):1468–1481. doi: 10.1080/15548627.2019.1687215

Figure 2.

Figure 2.

Nicotinamide mononucleotide (NMN) reactivated autophagy in Ndufc2-silenced A10 cells. (A-C) Representative western blots of LC3 and SQSTM1 with densitometric analyses in Ndufc2-silenced (siRNA) A10 cells treated with sodium chloride (NaCl) (n = 4). (D) NAD+:NADH ratio in cells with Ndufc2 silencing, treated or not with NMN (n = 3–7). (E-F) Representative western blot of LC3 and corresponding densitometric analysis in Ndufc2-depleted A10 cells treated with NaCl and exposed to NMN (n = 3). (G-H) Representative western blot of COX4I1 and corresponding densitometric analysis in Ndufc2-depleted A10 cells treated with NaCl and exposed to NMN (n = 3). (I) Mitochondrial DNA content (mtDNA) in Ndufc2-silenced cells exposed to NaCl and treated with NMN (n = 4). CTR indicates non-silenced and untreated cells. Results are presented as mean values ± SEM; *p < 0.05 **p < 0.01 ***p < 0.001 for the indicated comparisons.