Skip to main content
. 2019 Nov 12;16(8):1468–1481. doi: 10.1080/15548627.2019.1687215

Figure 3.

Figure 3.

NMN improved mitochondrial function and cell viability in vitro. (A) Evaluation of ultrastructural damage in mitochondria from A10 cells with Ndufc2 knockdown; representative micrographs of mitochondria (left) and graphical representation of the ultrastructural damage in either untreated or treated A10 cells (n = 3). Legend: Nu, nucleus; NM, nuclear membrane, PM, plasma membrane; rER, rough endoplasmic reticulum; Gx, grade of mitochondrial (Mt) damage; IMM, inner mitochondrial membrane; OMM, outer mitochondrial membrane. (B) Fluorescence microscope analysis of mitochondrial membrane potential (ΔΨm) levels through JC1 dye (n = 3); representative images (left) and corresponding quantification (right) are shown. (C) FACS analysis in A10 cells with Ndufc2 knockdown treated with NaCl without or with NMN (n = 3); CTR indicates non-silenced and untreated cells. Results are presented as mean values ± SEM; *p < 0.05 **p < 0.01 ***p < 0.001; ns, not significant for the indicated comparisons.