Figure 6.

SLC16A1-AS1 silencing inhibits cell proliferation and arrest cell cycle of OSCC cells in vitro.

a. The mRNA expression of commonly used cell proliferation markers between low- and high-SLC16A1-AS1 groups indicating high-SLC16A1-AS1 is associated with high proliferation activity. b. The protein expression of cell proliferation marker PCNA in low- and high-SLC16A1-AS1 group. c. qRT-PCR analysis of the RNA level of SLC16A1-AS1 in cells transfected with NC or SLC16A1-AS1 smart silencer. d,e. Cells transfected with NC or SLC16A1-AS1 smart silencer were examined by CCK8 assays F. Western blot of the protein level of cyclin D1 in cells transfected with NC or SLC16A1-AS1 smart silencer G. Cells with transfection of NC or SLC16A1-AS1 smart silencer were examined by colony formation assay. H. Flow cytometry system analysis was used to detect the phase of the cell cycle in cells transfected with NC or SLC16A1-AS1 smart silencer.