Figure 1.

DUSP3 lof reduces cell proliferation and reorganizes cell cycle distribution after genotoxic stress. (A) Control cells (non-silenced, NS) or cells silenced for DUSP3 (shDUSP3) were plated at day “0” and exposed to UVC irradiation (4 J/m2) 24 hours after (day “1”); then cells were collected and counted in the subsequent days. Graphs are average of three independent experiments ± standard deviation. (B) MRC-5, XPA, XPC or XPV shDUSP3 cells, or their control (NS), were submitted to UVC radiation (6 J/m2) treatments and collected at 0ʹ and 24 hours later. Cells were fixed, permeabilized, stained with propidium iodide and analyzed in a cytometer. Graphs represent average of three independent experiments ± standard deviation.