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. 2020 Jun 28;19(15):1884–1898. doi: 10.1080/15384101.2020.1779471

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 2. — BMI1 is required for cell survival and testosterone production in MLTC-1 cells. (a) Western blot results for MLTC-1 cells treated with 10 μM PTC-209 for 48 h. Sample number = 3. (b) Quantification of a. (c) MTT assay for MLTC-1 cells treated with DMSO (Ctr) or 10 μM PTC-209 for the indicated time points. Sample number = 6. (d) Testosterone levels in MLTC-1 cells treated with DMSO (Ctr) or 10 μM PTC-209 for 48 h. Sample number = 6. * p < 0.05; **p < 0.01; ***p < 0.001. For (b,d), Student’s t-test; for (c), one-way ANOVA.