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. 2020 Jul 17;19(16):2063–2073. doi: 10.1080/15384101.2020.1792686

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Figure 8. — Regulation of Notch pathway after transfection with Notch1 overexpression plasmids in MM cells. mRNA expressions of Notch pathway-related factors (including Notch1, Jagged1, Jagged2, Hes1) after transfection with Notch1 overexpression plasmids in RPMI-8226 cells (a). Protein expressions of Notch pathway-related factors (including Notch1, Jagged1, Jagged2, Hes1) after transfection with Notch1 overexpression plasmids in RPMI-8226 cells (b, c). mRNA expressions of Notch pathway-related factors (including Notch1, Jagged1, Jagged2, Hes1) after transfection with Notch1 overexpression plasmids in U266 cells (d). Protein expressions of Notch pathway-related factors (including Notch1, Jagged1, Jagged2, Hes1) after transfection with Notch1 overexpression plasmids in U266 cells (e, f). Comparison between two groups was determined by t-test. P value < 0.05 was considered significant. **, P < 0.01; ***, P < 0.001; NS, no significance. MM, multiple myeloma. C-Caspase 3, Cleaved Caspase 3; Bcl-2, B-cell lymphoma-2.