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. 2020 Jun 28;19(15):1952–1968. doi: 10.1080/15384101.2020.1785724

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 1. — ATAD5 is localized at the centrosomes in an RFC independent manner.

(a) U2OS cells and (b) NIH3T3 cells were fixed at asynchronous condition for immunostaining. Bar, 5 μm. (c) The wild type (Atad5^+/+) mouse embryonic stem cells (mESCs) and Atad5 knock out (Atad5^−/-) mESCs were fixed for immunostaining. Bar, 5 μm. The inset shows high-magnification image of the centrosome. Bar, 2 μm. (d) U2OS cells were transfected with a DNA vector expressing mNeonGreen-fused ATAD5 N-terminal fragment (1–693 amino acids). After 48 h of transfection, cells were fixed for immunostaining as indicated. Bar, 5 μm. (e) HeLa cells stably expressing GFP-tagged Centrin1 (HeLa_Centrin1-GFP) were fixed at asynchronous condition for immunostaining. The arrow indicates a centrosome. Bar, 5 μm. The inset shows high-magnification image. Bar, 1 μm.