Figure 3.

Centrosomes are over-duplicated in ATAD5-depleted cells.

(a-c, e-g) 48 h after transfection of siRNA or cDNA as indicated, cells were immunostained with an anti-γ-tubulin antibody. The percentage of cells with more than two centrosomes was displayed. Three independent experiments were performed and at least 300 cells were counted per experiment. Values represent mean ± SEM. Statistics: paired t-test. *, p ≤ 0.05, **, p ≤ 0.01, ns, not significant. (a-c, g) The right panels of graphs show immunoblot results from whole cell extracts prepared 48 h after transfection. (d, e) Cells were treated with 2 ug/mL aphidicolin (APH) for 24 h to arrest cells at the G1/S phase boundary. (d) Cells were fixed and DNA was stained with propidium iodide for cell cycle analysis.