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. 2020 Jun 28;19(15):1899–1916. doi: 10.1080/15384101.2020.1782036

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This work was authored as part of the Contributor's official duties as an Employee of the United States Government and is therefore a work of the United States Government. In accordance with 17 U.S.C. 105, no copyright protection is available for such works under U.S. Law.

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Figure 4. — RanBP1 is dispensable for cell growth and nucleo-cytoplasmic transport of a model substrate. (a) Crystal violet assay of HCT116^{RanBP1-μAID/HA} and parental HCT116 cells grown with or without Auxin (1 mM) for 4 d. Quantification of the number of cells are shown on the right. (b) Left: HCT116^{RanBP1-μAID/HA} cells, expressing a model NLS-NES-containing substrate (NLS-mCherry-LEXY). In this system, exposure of cells to UV light induces nuclear export, while switching off light induces nuclear import. White circle indicates the region of fluorescent intensity measurements. Right: Quantification of nucleocytoplasmic transport in HCT116^{RanBP1-μAID/HA} cells without (n = 6) and with 1 mM Auxin (n = 4). Note similar dynamics of both nuclear import and export in cells with or without RanBP1. N.s., non-significant (t-test).