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. 2020 Jun 28;19(15):1899–1916. doi: 10.1080/15384101.2020.1782036

Figure 7.

Figure 7.

RanBP1 regulates mitotic HURP localization. (a) Immuno-staining with HURP and Tubulin antibodies in HCT116RCC1-µAID−3xFLAG, HCT116RanGAP1−3mAID and HCT116RanBP1-µAID-HA cells treated with or without 1 mM Auxin for 5, 2 and 3 h, respectively. White dashed lines represent the length of HURP signal (top row). Bottom: graphical representations of the expected distribution of the RanGTP gradient under indicated conditions. (b) Quantification of HURP signal normalized by Tubulin staining on the spindle in (A). Bars indicate mean ± SD from a total of 10 cells in more than 3 independent experiments. *** (p < 0.001, t-test). Scale bars = 10 μm.