Figure 2.

Rb2 inhibited adipocyte pyroptosis in epididymal adipose tissue (EATs). (a) Increased mRNA expression levels of caspase 1, ASC, NLRP3, IL-1β and GSDMD in HFD-fed mice EAT, which can be ameliorated by Rb2. (b) Pro-caspase 1 and total caspase 1 levels, ratio of pro-caspase 1/caspase 1. (c) ASC, NLRP3 and GSDMD levels. (d) ASC staining from EAT secetion. N = 5 per group. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01 compared with CD+PBS group; ^#P < 0.05, ^##P < 0.01 compared with HFD+PBS group. GSDMD is a quintessential mediator of pyroptosis