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. 2019 Sep 4;16(6):1111–1129. doi: 10.1080/15548627.2019.1659613

Figure 8.

Figure 8.

CAVIN1 knockdown promotes autophagic degradation of CAV1. (A, B) HUVECs were transfected with CAVIN1 siRNA (20 nM) or scrambled siRNA (20 nM) for 24 h, followed by transfection with ATG5 siRNA (20 nM) or scrambled siRNA (20 nM) for another 24 h. (C-D) HUVECs were transfected with CAVIN1 siRNA or scrambled siRNA for 24 h, followed by treatment with bafilomycin A1 (100 nM, 24 h). The expression of specific proteins was analyzed by immunoblotting assay. Representative western blotting of the indicated proteins (A, C) and summary bar graph showing the expression of the indicated proteins (B, D). * p < 0.05 versus scrambled siRNA; † p < 0.05 versus CAVIN1 siRNA (n = 3). (E-F) HUVECs were transfected with GFP-CAV1 plasmids for 24 h, and then transfected with scrambled siRNA (20 nM) or CAVIN1 siRNA (20 nM) for 48 h, followed by LysoTracker treatment (100 nM, 1 h). Blots images (E) and fluorescent images (F) are representative of at least 3 independent experiments. Scale bars: 15 μm.