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. 2020 Aug 24;3(4):276–283. doi: 10.1089/crispr.2020.0021

FIG. 3.

FIG. 3.

(A) Western blot of AAV-transduced samples targeting VEGF. (B) Western blot of AAV-transduced samples targeting RHO. (C) Quantitative analysis of band densities normalized to alpha-tubulin, relative to the expression of SaCas9 with U6 in the forward orientation (VEGF-Rev = 0.38 [95% CI 0.23–0.52], p = 0.0001, n = 5; RHO-Rev = 0.70 [95% CI 0.54–0.86], p = 0.001, n = 5). Two-way unpaired ANOVA with Šídák's multiple comparison testing. NTC = non transfected control; rSaCas9 = 100 ng recombinant SaCas9 protein.