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. 2020 Aug 24;3(4):276–283. doi: 10.1089/crispr.2020.0021

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Copyright 2020, Mary Ann Liebert, Inc., publishers

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FIG. 4. — A model of the three possible arrangements of a dual-transgene containing Cas9 driven by a Pol-II promoter and a gRNA driven by a Pol-III promoter in a circular episomal monomer or concatemer. (A) With the gRNA and its promoter oriented in the forward direction, only one conformation of transgene concatemer results in promoter proximity. (B) In concatemers with the gRNA and its promoter oriented in the reverse direction, all conformations of the transgene concatemer result in proximity between the promoters.