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. 2020 Aug 24;3(4):299–313. doi: 10.1089/crispr.2020.0032

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© Thomas Fricke et al. 2020; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 2. — Microscopy of StCsm mediated EGFP knockdown in Tg(ddx4:ddx4-EGFP) fish. Fluorescence from Tg(ddx4:ddx4-EGFP) fish after injection with wildtype (wt) or mutant StCsm complexes are shown. The arrows indicate the location of primordial germ cells. Injection was done at the 1-cell embryo stage, observations were at 3 hpf, 1 dpf, 2 dpf, and 5 dpf. The scale bar represents 1 mm. Boxes in the top row show the region of the embryo magnified in panels below. Arrows point to germ cells.