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. 2020 Aug 24;3(4):299–313. doi: 10.1089/crispr.2020.0032

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© Thomas Fricke et al. 2020; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 3. — Quantification of EGFP knockdown efficiency by fluorescence-activated cell sorting analysis. Enhanced green fluorescent protein fluorescence in (A) Tg(ddx4:ddx4-EGFP), (B) Tg(Xla.Eef1a1:mlsEGFP), (C) Tg(nkx2.5:EGFP), (D) Tg(myl7:GFP) and (E) Tg(fli1:EGFP) fish was quantified 1 dpf and 2 dpf by flow cytometry of minced and trypsinized embryos. For Tg(ddx4:ddx4-EGFP), Tg(nkx2.5:EGFP) and Tg(myl7:GFP) embryos, the number of highly fluorescent cells (at least 50-fold background fluorescence) was counted. Results are from 3 independent experiments; error bars represent one standard deviation. *P < 0.05 as compared with respective controls, ***P < 0.001 as compared with respective controls. Data are represented as mean ± SEM.