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. 2020 Aug 24;3(4):299–313. doi: 10.1089/crispr.2020.0032

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© Thomas Fricke et al. 2020; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 4. — Knockdown of endogenous tdgf1. (A) Dose-dependent penetrance and lethality were assessed after injection of dDNase StCsm(tdgf1^{167,174,154,181}). Different amounts of dDNase StCsm(tdgf1^{167,174,154,181}) were injected in a constant volume of 1 nL at the 1-cell stage. Phenotypes were screened after 24 hpf on the basis of morphological changes. Data represented as mean ± SEM. (B) Bar plot comparing the efficiency of different StCsm complexes. 1.5 ng of either wt, dRNase or dDNase StCsm(tdgf1^{167,174,154,181}) complexes in 1 nL volume was injected in the same clutch of embryos. Results from injections in five different clutches of embryos are presented. Data represented as mean ± SEM. (C) Lateral and ventral images of the injected embryos after 24 hpf. Arrows indicate position of eye or eyes. Scale bar represents 1 mm.